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microarray processing analysis  (Thermo Fisher)


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    Structured Review

    Thermo Fisher microarray processing analysis
    Microarray Processing Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+processing+and+analysis/pm35077654-102-1-0
    Average 90 stars, based on 1 article reviews
    microarray processing analysis - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Role of LFA-1 integrin in the control of a lymphocytic choriomeningitis virus (LCMV) infection
    Article Snippet: RNA processing and microarray analysis with the Affymetrix MoGene 1.0 ST array was prepared according to standard operating procedures of the ImmGen Project (http://www.immgen.org/Protocols/Total RNA Extraction with Trizol.pdf; http://www.immgen.org/Protocols/ImmGen QC Documentation_ALL-DataGeneration_0612.pdf).

    Article Title: Identification of new target proteins of a Urotensin-II receptor antagonist using transcriptome-based drug repositioning approach
    Article Snippet: Microarray data export processing and basic analysis were performed using the Affymetrix ® GeneChip Command Console® Software version 6.0 + (AGCC, www.thermofisher.com/kr/ko/home/life-science/microarray-analysis/microarray-analysis-instruments-software-services/microarray-analysis-software/affymetrix-genechip-command-console-software.html ).

    Article Title: Microarray-based IgE detection in tears of patients with vernal keratoconjunctivitis.
    Article Snippet: Background: A specific allergen sensitization can be demonstrated in approximately half of the vernal keratoconjunctivitis (VKC) patients by conventional allergic tests.. The measurement of specific IgE in tears using a multiplex allergen microarray may offer advantages to identify local sensitization to a specific allergen.. Methods: In spring–summer 2011, serum and tears samples were collected from 10 active VKC patients (three females, seven males) and 10 age-matched normal subjects.

    Article Title: Hypoxic exosomes facilitate angiogenesis and metastasis in esophageal squamous cell carcinoma through altering the phenotype and transcriptome of endothelial cells
    Article Snippet: Quantile normalization and subsequent data processing were performed using Applied BiosystemsTM Transcriptome Analysis Console (TAC) Software (Affymetrix, USA).

    Article Title: Methods for treating polyps
    Article Snippet: 3) Microarray Processing and Analysis Preparation of cRNA, hybridization to the Affymetrix HG-U133 plus 2.0 GeneChip (Affymetrix, Santa Clara, Calif.), and scanning of the chip was performed according to the manufacturer's protocol in a core facility at the Harvard Medical School—Partners Healthcare Center for Genetics and Genomics (Cambridge, Mass.).

    Fluorescence:

    Article Title: Validation of a multiplex chip-based assay for the detection of autoantibodies against citrullinated peptides
    Article Snippet: The slides were incubated for 30 minutes and then washed again before they were scanned by using a laser scanner (LuxScan 10K; CapitalBio, Beijing, China). .. Fluorescence intensities were recorded, and data processing was performed by using the Microarray Image Analysis software (Phadia). ..

    Microarray:

    Article Title: Validation of a multiplex chip-based assay for the detection of autoantibodies against citrullinated peptides
    Article Snippet: The slides were incubated for 30 minutes and then washed again before they were scanned by using a laser scanner (LuxScan 10K; CapitalBio, Beijing, China). .. Fluorescence intensities were recorded, and data processing was performed by using the Microarray Image Analysis software (Phadia). ..

    Article Title: EMP1, a novel poor prognostic factor in pediatric leukemia regulates prednisolone resistance, cell proliferation, migration and adhesion.
    Article Snippet: The Affymetrix One-Cycle cDNA Synthesis kit (Santa Clara, CA, USA) and the GeneChip IVT Labeling kit (Santa Clara, CA, USA) were used to synthesize cRNA. .. RNA processing and hybridization to the Affymetrix U133 Plus 2.0 GeneChip oligonucleotide microarray were performed according to the manufacturer’s protocol. .. Gene-expression values were calculated with Affymetrix Microarray Suite version 5.0.

    Article Title: Post-translational modification of OCT4 in breast cancer tumorigenesis
    Article Snippet: Gene expression in the examined cell lines was analyzed using high-density oligonucleotide microarrays containing 20,889 transcripts (HG-U133 Plus 2.0; Affymetrix). .. Target preparation and microarray processing were performed as described in Affymetrix GeneChip expression analysis manual. .. GeneChip analysis was performed using Affymetrix GeneChip manual with Microarray Analysis Suite 5.0, Data Mining Tool 2.0, and Microarray Database software.

    Software:

    Article Title: Validation of a multiplex chip-based assay for the detection of autoantibodies against citrullinated peptides
    Article Snippet: The slides were incubated for 30 minutes and then washed again before they were scanned by using a laser scanner (LuxScan 10K; CapitalBio, Beijing, China). .. Fluorescence intensities were recorded, and data processing was performed by using the Microarray Image Analysis software (Phadia). ..

    Hybridization:

    Article Title: EMP1, a novel poor prognostic factor in pediatric leukemia regulates prednisolone resistance, cell proliferation, migration and adhesion.
    Article Snippet: The Affymetrix One-Cycle cDNA Synthesis kit (Santa Clara, CA, USA) and the GeneChip IVT Labeling kit (Santa Clara, CA, USA) were used to synthesize cRNA. .. RNA processing and hybridization to the Affymetrix U133 Plus 2.0 GeneChip oligonucleotide microarray were performed according to the manufacturer’s protocol. .. Gene-expression values were calculated with Affymetrix Microarray Suite version 5.0.

    Expressing:

    Article Title: Post-translational modification of OCT4 in breast cancer tumorigenesis
    Article Snippet: Gene expression in the examined cell lines was analyzed using high-density oligonucleotide microarrays containing 20,889 transcripts (HG-U133 Plus 2.0; Affymetrix). .. Target preparation and microarray processing were performed as described in Affymetrix GeneChip expression analysis manual. .. GeneChip analysis was performed using Affymetrix GeneChip manual with Microarray Analysis Suite 5.0, Data Mining Tool 2.0, and Microarray Database software.



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    Ribobio co microarray processing and analysis for mirs
    Chronic normobaric hypoxia (CNH) exposure induces pulmonary arterial hypertension (PAH) and upregulates miR-335-3p expression in the lungs of mice. CNH treatment significantly increased RVSP (A) and RV/(LV+S) (B) (N=5-8 per group). (C) Heatmap and sample clustering analysis of the differential expressed miRs in the lungs of mice (N=4 per group). Each row represents a miR and each column represents a sample. (D) <t>Microarray</t> and (E) quantitative RT-PCR analysis of miR-335-3p expression in the lungs of mice. (F) Correlation analysis between miR-335-3p and RVSP, and (G) Correlation analysis between miR-335-3p and RV/(LV+S) (N=4 per group). Values shown are means±s.e.m. ** p <0.01, *** p <0.001 vs. Normoxia.
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    Image Search Results


    Chronic normobaric hypoxia (CNH) exposure induces pulmonary arterial hypertension (PAH) and upregulates miR-335-3p expression in the lungs of mice. CNH treatment significantly increased RVSP (A) and RV/(LV+S) (B) (N=5-8 per group). (C) Heatmap and sample clustering analysis of the differential expressed miRs in the lungs of mice (N=4 per group). Each row represents a miR and each column represents a sample. (D) Microarray and (E) quantitative RT-PCR analysis of miR-335-3p expression in the lungs of mice. (F) Correlation analysis between miR-335-3p and RVSP, and (G) Correlation analysis between miR-335-3p and RV/(LV+S) (N=4 per group). Values shown are means±s.e.m. ** p <0.01, *** p <0.001 vs. Normoxia.

    Journal: International Journal of Biological Sciences

    Article Title: Upregulation of miR-335-3p by NF-κB Transcriptional Regulation Contributes to the Induction of Pulmonary Arterial Hypertension via APJ during Hypoxia

    doi: 10.7150/ijbs.34517

    Figure Lengend Snippet: Chronic normobaric hypoxia (CNH) exposure induces pulmonary arterial hypertension (PAH) and upregulates miR-335-3p expression in the lungs of mice. CNH treatment significantly increased RVSP (A) and RV/(LV+S) (B) (N=5-8 per group). (C) Heatmap and sample clustering analysis of the differential expressed miRs in the lungs of mice (N=4 per group). Each row represents a miR and each column represents a sample. (D) Microarray and (E) quantitative RT-PCR analysis of miR-335-3p expression in the lungs of mice. (F) Correlation analysis between miR-335-3p and RVSP, and (G) Correlation analysis between miR-335-3p and RV/(LV+S) (N=4 per group). Values shown are means±s.e.m. ** p <0.01, *** p <0.001 vs. Normoxia.

    Article Snippet: Microarray processing and analysis for miRs was performed by the Ribobio Co., Ltd. (Guangzhou, China).

    Techniques: Expressing, Microarray, Quantitative RT-PCR